Simple Science: Some Cave Research

In the Northup lab, I study microbiology in cave environments. My project is on nutrient cycling within a few New Mexican caves. Caves offer a great laboratory to study bacteria without the influences of the elements, such as solar radiation and wind erosion. Inside caves, bacteria can live off of limited nutrients found on the cave walls or floor. We know bacteria help facilitate the break down of dead organisms, but in caves some bacteria can break down rock and promote cave enlargement.

Let me take a step back—way back. Bacteria have dominated Earth since life first evolved between 3.5-4 bya. They were alone for 3 billion years until multicellular life evolves 3 billion years later (2.1 bya). Just to add perspective,  Homo sapiens evolved 0.2 mya. Bacteria are the most diverse domain of life and can be found almost every possible place on Earth, such as deep-sea thermal vents and clouds. The study of this microbial diversity and how they interact with the environment is called microbial ecology.

Today the Northup lab is interested in metagenomics of caves, which is a tool to capture the entire microbial community’s genetic material from environmental samples. Jason Kimble and Ara Kooser both study nitrogen fixation genes among the communities, including the nifH and amoA genes. My research hasn’t moved to metagenomics yet, I’m still looking at the presence of enzymes responsible for decomposition. But with their shit-load of data, I can possibly mine to look for genes responsible for making the enzymes I’m interested in.

N-Cycling Genes in Caves: amoA & nifH Genes in Azorean Lava Caves

My mentor, Diana Northup, studies lava caves in Azores, Portugal. Lava caves are great places to study bacterial communities, which may produce antibiotics; and are model systems for Martian caves that may host microbial life.

Hathaway et al. (2014) examined Azorean lava caves for two genes that encode for enzymes responsible for nitrogen transformation. Nitrogen is a nutrient critical for living organisms. The nitrogen cycle allows for the conversion of N2 into bioavailable forms, such as ammonia (NH3). The two genes were nitrogen fixation (nifH) and ammonia monooxygenase (amoA); they’re responsible for N-fixation and nitrficiation (fig. 1).

Nitrogen Cycle & Enzymes
Figure 1. Nitrogen Cycle & Enzymes

nifH encodes for nitrogenase, which helps convert Nto NH3. Ammonia can be a source of energy for chemolithotrophic organisms; they obtain energy from redox reactions of inorganic compounds. amoA converts NH3 to hydroylamine (NH2OH)– the limiting step in nitrification. They collected samples from bacterial mats in 11 lava caves that were beneath different land use: pasture, forest, and urban. Prior to this study, the genes were not studied in caves along with analyzing soil and water chemistry.

Hathaway found no difference among caves under different land use for water and soil chemistries, but lava caves below forested land had greater diversity of ammonia-oxidizing bacteria and an abundance of nifH genes. They suggested there might be other abiotic influences over bacterial communities. However, the study didn’t explain the statistics and did not determine if the genes are being expressed.  But this was the first study of its kind.


Article:
Jennifer J. Marshall Hathaway , Robert L. Sinsabaugh , Maria De Lurdes N. E. Dapkevicius & Diana E. Northup (2014) Diversity of Ammonia Oxidation (amoA) and Nitrogen Fixation (nifH) Genes in Lava Caves of Terceira, Azores, Portugal, Geomicrobiology Journal, 31:3, 221-235, DOI: 10.1080/01490451.2012.752424

Intro & Goals

Hello! I’m Noelle, a 3rd year PhD student that enjoys writing and talking to the public about science. I’m still finding my voice and learning how to share my love for caves & soils without being cheesy.

My comprehensives are coming up this autumn and I will be using this space as a way to summarize my thoughts, journal articles, and books. I may pepper in postings that are non-science related (NSR).

Goals:

  • At least 3 entries per week: Monday, Wednesday, and Friday posts
  • One entry per book or article(s)- articles if they’re related so I may connect them together
  • Entry shall be less than 250 words

Topics

  • Cave microbiology
  • Soil ecology/ microbiology/ biogeochemistry
  • Plant-microbe interactions
  • Locations: Arches; Duke Forest; Chihuahua Desert; Spider Cave, NM; Lechuguilla Cave, NM

* Me collecting moonmilk in a cave. Photo courtesy of Kenneth Ingham.